E-ISSN 2277-338X | ISSN 2320-4664
 

Original Research 


Comparative evaluation of Mycobacterium tuberculosis drug susceptibility testing between Direct Nitrate Reductase assay and Direct Proportion Method

Neelu Jain, Nitin Jain, Vaishali Jain.

Abstract
Background: Drug resistance – multi-drug resistant (MDR) and extensive drug resistant (XDR) in tuberculosis (TB) is a matter of great concern for TB control programs. There is concern and need for early diagnosis of these multi-drug-resistant strains for better treatment.

Objective: To compare the usefulness of Nitrate Reductase Assay (NRA) and direct proportion as a tool for rapid and accurate detection of resistance to first line anti-tubercular drugs.

Material and Methods: A total of 120 sputum-positive AFB smears of pulmonary tuberculosis (PTB) patients were selected for the study. The samples were processed by NRA and direct proportion for assessment of drug susceptibility testing to first line anti-tubercular drugs.

Results: The sensitivity of NRA was 100%, 92.30%, 81.81%, and 72.72 % for RIF, INH, EMB, and STR, respectively. The specificity of NRA was 100%, 100%, 96.33%, and 89.79% for RIF, INH, EMB, and STR, respectively. The performance of NRA susceptibility testing was rapid and the median time of obtaining results was shorter using NRA (10 days) as compared to PM (28 days).

Conclusion: Direct NRA is simple, easy to perform, rapid, relatively less expensive, without requirement of expensive reagents and sophisticated equipments. It is useful tool suitable for early determination, first line anti-tubercular drugs namely rifampicin, isoniazid, ethambutol, and streptomycin with excellent concordance for INH and RIF resistance and relatively low accuracy for streptomycin, with good sensitivity and specificity.

Key words: Tuberculosis, multidrug resistant, Nitrate Reductase Assay


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Neelu Jain
Articles by Nitin Jain
Articles by Vaishali Jain
on Google
on Google Scholar
Article Statistics
 Viewed: 1936
Downloaded: 404
Cited: 0

How to Cite this Article
Pubmed Style

Neelu Jain, Nitin Jain, Vaishali Jain. Comparative evaluation of Mycobacterium tuberculosis drug susceptibility testing between Direct Nitrate Reductase assay and Direct Proportion Method. Int J Med Sci Public Health. 2017; 6(1): 86-90. doi:10.5455/ijmsph.2017.22062016563


Web Style

Neelu Jain, Nitin Jain, Vaishali Jain. Comparative evaluation of Mycobacterium tuberculosis drug susceptibility testing between Direct Nitrate Reductase assay and Direct Proportion Method. http://www.ijmsph.com/?mno=234719 [Access: April 23, 2019]. doi:10.5455/ijmsph.2017.22062016563


AMA (American Medical Association) Style

Neelu Jain, Nitin Jain, Vaishali Jain. Comparative evaluation of Mycobacterium tuberculosis drug susceptibility testing between Direct Nitrate Reductase assay and Direct Proportion Method. Int J Med Sci Public Health. 2017; 6(1): 86-90. doi:10.5455/ijmsph.2017.22062016563



Vancouver/ICMJE Style

Neelu Jain, Nitin Jain, Vaishali Jain. Comparative evaluation of Mycobacterium tuberculosis drug susceptibility testing between Direct Nitrate Reductase assay and Direct Proportion Method. Int J Med Sci Public Health. (2017), [cited April 23, 2019]; 6(1): 86-90. doi:10.5455/ijmsph.2017.22062016563



Harvard Style

Neelu Jain, Nitin Jain, Vaishali Jain (2017) Comparative evaluation of Mycobacterium tuberculosis drug susceptibility testing between Direct Nitrate Reductase assay and Direct Proportion Method. Int J Med Sci Public Health, 6 (1), 86-90. doi:10.5455/ijmsph.2017.22062016563



Turabian Style

Neelu Jain, Nitin Jain, Vaishali Jain. 2017. Comparative evaluation of Mycobacterium tuberculosis drug susceptibility testing between Direct Nitrate Reductase assay and Direct Proportion Method. International Journal of Medical Science and Public Health, 6 (1), 86-90. doi:10.5455/ijmsph.2017.22062016563



Chicago Style

Neelu Jain, Nitin Jain, Vaishali Jain. "Comparative evaluation of Mycobacterium tuberculosis drug susceptibility testing between Direct Nitrate Reductase assay and Direct Proportion Method." International Journal of Medical Science and Public Health 6 (2017), 86-90. doi:10.5455/ijmsph.2017.22062016563



MLA (The Modern Language Association) Style

Neelu Jain, Nitin Jain, Vaishali Jain. "Comparative evaluation of Mycobacterium tuberculosis drug susceptibility testing between Direct Nitrate Reductase assay and Direct Proportion Method." International Journal of Medical Science and Public Health 6.1 (2017), 86-90. Print. doi:10.5455/ijmsph.2017.22062016563



APA (American Psychological Association) Style

Neelu Jain, Nitin Jain, Vaishali Jain (2017) Comparative evaluation of Mycobacterium tuberculosis drug susceptibility testing between Direct Nitrate Reductase assay and Direct Proportion Method. International Journal of Medical Science and Public Health, 6 (1), 86-90. doi:10.5455/ijmsph.2017.22062016563





Most Viewed Articles
  • Management of Appendicular Lump: Early exploration Vs conservative management
    Chandra Prakash Pandey, Ram Chandra Kesharwani, Chandra Gopal Singh Chauhan, Manmohan Krishna Pandey, Purnima Mittra, Pramod Kumar, Arshad Raza
    Int J Med Sci Public Health. 2013; 2(4): 1046-1049
    » Abstract & References » doi: 10.5455/ijmsph.2013.230820131

  • Burden of non communicable diseases in India: Setting priority for action
    Kalpa Sharma
    Int J Med Sci Public Health. 2013; 2(1): 7-11
    » Abstract & References » doi: 10.5455/ijmsph.2013.2.7-11

  • Internet addiction among professional courses students: A study from central India
    Arvind Sharma, Rupesh Sahu, Pradeep Kumar Kasar, Richa Sharma
    Int J Med Sci Public Health. 2014; 3(9): 1069-1073
    » Abstract & References » doi: 10.5455/ijmsph.2014.180620142

  • Diabetic nephropathy: Progression and pathophysiology
    Deepak N Parchwani, Amit A Upadhyah
    Int J Med Sci Public Health. 2012; 1(2): 59-70
    » Abstract & References » doi: 10.5455/ijmsph.2012.1.59-70

  • Elevated levels of serum uric acid, creatinine or urea in preeclamptic women
    Magna Manjareeka, Sitikantha Nanda
    Int J Med Sci Public Health. 2013; 2(1): 43-47
    » Abstract & References » doi: 10.5455/ijmsph.2013.2.43-47

  • Maternal and fetal outcome in oligohydramnios- Study of 100 cases
    Krishna Jagatia, Nisha Singh, Sachin Patel
    Int J Med Sci Public Health. 2013; 2(3): 724-727
    » Abstract & References » doi: 10.5455/ijmsph.2013.070520132

  • Zinc: The trace element of major importance in human nutrition and health
    Jayant D Deshpande, Mohini M Joshi, Purushottam A Giri
    Int J Med Sci Public Health. 2013; 2(1): 1-6
    » Abstract & References » doi: 10.5455/ijmsph.2013.2.1-6

  • Knowledge, Attitude, and Practices (KAP) study on HIV / AIDS among HIV patients, care givers and general population in north-eastern part of India.
    L P Meena, S K Pandey, M Rai, Anju Bharti, Shyam Sunder
    Int J Med Sci Public Health. 2013; 2(1): 36-42
    » Abstract & References » doi: 10.5455/ijmsph.2013.2.36-42

  • Patient satisfaction about health care services: A cross sectional study of patients who visit the outpatient department of a civil hospital at Surendranagar, Gujarat
    Krupal Joshi, Kishor Sochaliya, Shyamal Purani, Girija Kartha
    Int J Med Sci Public Health. 2013; 2(3): 659-663
    » Abstract & References » doi: 10.5455/ijmsph.2013.250420131

  • Prevalence and pattern of substance abuse among school childern in nothern India: A rapid assesment study
    Syed Shuja Qadri, RKD Goel, Jagjeet Singh, Surendra Kumar Ahluwalia, Rambha Pathak, Humaira Bashir
    Int J Med Sci Public Health. 2013; 2(2): 273-282
    » Abstract & References » doi: 10.5455/ijmsph.2013.2.271-280

  • Most Downloaded
  • Minor disorder of pregnancy and its home management
    Lata Gururani, Atul Kumar, Gomathi Mahalingam
    Int J Med Sci Public Health. 2016; 5(4): 684-687
    » Abstract & References » doi: 10.5455/ijmsph.2016.03082015111

  • Association between serum ferritin and thyroid hormone profile in hypothyroidism
    Ashuma Sachdeva, Veena Singh, Isha Malik, Prasanta Saha Roy, Himanshu Madaan, Rajesh Nair
    Int J Med Sci Public Health. 2015; 4(6): 863-865
    » Abstract & References » doi: 10.5455/ijmsph.2015.24012015174

  • Internet addiction among professional courses students: A study from central India
    Arvind Sharma, Rupesh Sahu, Pradeep Kumar Kasar, Richa Sharma
    Int J Med Sci Public Health. 2014; 3(9): 1069-1073
    » Abstract & References » doi: 10.5455/ijmsph.2014.180620142

  • Effect of sleep deprivation on the attitude and performance of medical students, Riyadh, Saudi Arabia
    Hussein Saad Amin, Ibrahim Salamah Almazroua, Abdullah Sulaiman Alsahlan, Mohammed Abdullah Alrishan, Houssam Mourad Elmourad, Mohammed Mansour Alotaibi, Abdulaziz Ibrahim Almohaisin
    Int J Med Sci Public Health. 2016; 5(3): 575-580
    » Abstract & References » doi: 10.5455/ijmsph.2016.17112015207

  • Management of Appendicular Lump: Early exploration Vs conservative management
    Chandra Prakash Pandey, Ram Chandra Kesharwani, Chandra Gopal Singh Chauhan, Manmohan Krishna Pandey, Purnima Mittra, Pramod Kumar, Arshad Raza
    Int J Med Sci Public Health. 2013; 2(4): 1046-1049
    » Abstract & References » doi: 10.5455/ijmsph.2013.230820131

  • Epidemiology and prevalence of dermatological diseases among schoolchildren of Medak district, Telangana—a clinical survey
    Lakshmi Kumari Villa, Gopi Krishna
    Int J Med Sci Public Health. 2016; 5(7): 1475-1478
    » Abstract & References » doi: 10.5455/ijmsph.2016.28102015238

  • Gunthers with haemolytic anaemia extremly rare: A case report
    Nilesh Kumar, Ankur Varshney, Arvind Anand, Dibyaranjan Behera, Ravi Anand, Ashutosh Tiwari
    Int J Med Sci Public Health. 2013; 2(2): 472-474
    » Abstract & References » doi: 10.5455/ijmsph.2013.2.313-315

  • Diabetic nephropathy: Progression and pathophysiology
    Deepak N Parchwani, Amit A Upadhyah
    Int J Med Sci Public Health. 2012; 1(2): 59-70
    » Abstract & References » doi: 10.5455/ijmsph.2012.1.59-70

  • Patient satisfaction about health care services: A cross sectional study of patients who visit the outpatient department of a civil hospital at Surendranagar, Gujarat
    Krupal Joshi, Kishor Sochaliya, Shyamal Purani, Girija Kartha
    Int J Med Sci Public Health. 2013; 2(3): 659-663
    » Abstract & References » doi: 10.5455/ijmsph.2013.250420131

  • Burden of non communicable diseases in India: Setting priority for action
    Kalpa Sharma
    Int J Med Sci Public Health. 2013; 2(1): 7-11
    » Abstract & References » doi: 10.5455/ijmsph.2013.2.7-11

  • Most Cited Articles
  • Zinc: The trace element of major importance in human nutrition and health
    Jayant D Deshpande, Mohini M Joshi, Purushottam A Giri
    Int J Med Sci Public Health. 2013; 2(1): 1-6
    » Abstract & References » doi: 10.5455/ijmsph.2013.2.1-6
    Cited : 25 times [Click to see citing articles]

  • Burden of non communicable diseases in India: Setting priority for action
    Kalpa Sharma
    Int J Med Sci Public Health. 2013; 2(1): 7-11
    » Abstract & References » doi: 10.5455/ijmsph.2013.2.7-11
    Cited : 13 times [Click to see citing articles]

  • Design of fuzzy expert system for diagnosis of cardiac diseases
    Smita Sushil Sikchi , Sushil Sikchi , M S ALI
    Int J Med Sci Public Health. 2013; 2(1): 56-61
    » Abstract & References » doi: 10.5455/ijmsph.2013.2.56-61
    Cited : 9 times [Click to see citing articles]

  • Sugammadex associate persistant bradycardia
    Murat Bilgi, Abdullah Demirhan, Akcan Akkaya, Umit Yasar Tekelioglu, Hasan Kocoglu
    Int J Med Sci Public Health. 2014; 3(3): 372-374
    » Abstract & References » doi: 10.5455/ijmsph.2013.251220131
    Cited : 8 times [Click to see citing articles]

  • Migration and health-care access: Barriers to access government health services by migrant tribal community living in an eastern Indian city
    Suchismita Mishra, Yadlapalli S Kusuma, Bontha V Babu
    Int J Med Sci Public Health. 2015; 4(1): 101-108
    » Abstract & References » doi: 10.5455/ijmsph.2015.0110201424
    Cited : 7 times [Click to see citing articles]

  • Clinico-microbiological profile of women with vaginal discharge in a tertiary care hospital of northern India
    Varsha Chaudhary, Ved Prakesh, Kian Agarwal, Vijender Kumar Agrawal, Arun Singh, Sachin Pandey
    Int J Med Sci Public Health. 2012; 1(2): 75-80
    » Abstract & References » doi: 10.5455/ijmsph.2012.1.75-80
    Cited : 6 times [Click to see citing articles]

  • Correlation between obesity and cardio respiratory fitness
    Prabha Setty, BV Padmanabha, BR Doddamani
    Int J Med Sci Public Health. 2013; 2(2): 300-304
    » Abstract & References » doi: 10.5455/ijmsph.2013.2.298-302
    Cited : 6 times [Click to see citing articles]

  • Knowledge, Attitude, and Practices (KAP) study on HIV / AIDS among HIV patients, care givers and general population in north-eastern part of India.
    L P Meena, S K Pandey, M Rai, Anju Bharti, Shyam Sunder
    Int J Med Sci Public Health. 2013; 2(1): 36-42
    » Abstract & References » doi: 10.5455/ijmsph.2013.2.36-42
    Cited : 6 times [Click to see citing articles]

  • Non albicans Candida species: its isolation pattern, species distribution, virulence factors and antifungal susceptibility profile
    Sachin Chandrakant Deorukhkar, Santosh Saini
    Int J Med Sci Public Health. 2013; 2(3): 533-538
    » Abstract & References » doi: 10.5455/ijmsph.2013.080320131
    Cited : 6 times [Click to see citing articles]

  • Initial default among tuberculosis patients diagnosed in selected medical colleges of Puducherry: issues and possible interventions
    Divija Pillai, Anil J Purty, Stalin Prabakaran, Zile Singh, Govindarajan Soundappan, Velavan Anandan
    Int J Med Sci Public Health. 2015; 4(7): 957-960
    » Abstract & References » doi: 10.5455/ijmsph.2015.30012015196
    Cited : 6 times [Click to see citing articles]