E-ISSN 2277-338X | ISSN 2320-4664
 

Original Research 


Detection of extended spectrum β-lactamases (ESBLS) producers in clinical isolates

Bhavanarushi Sreekanth, Gogi Suresh Dattaraya.

Abstract
Background: Treatment of extended Spectrum β-lactamase (ESBL) producing gram negative bacilli is an increasing problem in hospitalized patients. Increasing resistance to third generation cephalosporins among gram negative bacilli has important therapeutic and clinical implications. ESBL producers significantly affect the course and outcome of infection and are associated with increased morbidity and mortality. The high proportion of ESBL producers and the outbreak of multi drug resistant gram negative bacilli are quite alarming and need expensive control measures. Appropriate laboratory detection is important to avoid inappropriate antimicrobial therapy.

Aims & Objectives: The present study was undertaken to investigate the high incidence of extended Spectrum β-lactamases (ESBLS) producers and their antibiotic susceptibility pattern.

Materials and Methods: A total of 228 GNB isolates, were studied for ESBL production. The isolates were screened for ESBL production by the double disc synergy test (DDST) as recommended by the Clinical Laboratory Standards Institute (CLSI). Antimicrobial susceptibility testing was done along with screening for ESBL production for commonly used antibiotics. Isolates which showed positive results were confirmed by phenotypic confirmatory disc diffusion test (PCDDT).

Results: Out of 228 GNB isolates studied, 102 (44.73%) isolates were positive for ESBL production by presumptive screening test, of which 84 (36.84%) isolates were confirmed by PCDDT. The isolates of Escherichia coli (71.6%) were the most common ESBL producers, followed by K. pneumoniae (22.6%) and others. Imipenem (94.45%) was the most active and reliable agent for the treatment of the infections which were caused by the ESBL producing organisms.

Conclusion: ESBL detection and antibiotic sensitivity testing should be undertaken routinely to avoid misuse of antibiotics and also prevent spread of these strains.

Key words: Extended Spectrum Beta Lactamases (ESBLS); Gram Negative Bacilli; 3rd Generation Cephalosporins


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Bhavanarushi Sreekanth
Articles by Gogi Suresh Dattaraya
on Google
on Google Scholar
Article Statistics
 Viewed: 3735
Downloaded: 853
Cited: 0


REFERENCES
1. Paterson DL, Bonomo RA. Extended-Spectrum β-lactamases. A Clinical Update. ClinMicrobiol Rev 2005;18:657–86. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
2. Andrews J. Detection of extended-spectrum β-lactamases (ESBLs) in E. coli and Klebsiella species. 2003. http://www.bsac.org.uk/uploads/Ecoliklebsiella.pdf
3. Kulkarini R, Vaishali D, Dnyaneshwari G, Aravind B. A study of Extended spectrum betalactamase (ESBL) producers in clinical isolates. Med J of west India 2013;41:18-22.
4. Gaurav D. Prevelance of Extended spectrum β-lactamase (ESBL) Producers among Gram Negative Bacilli from Various Clinical Isolates in a Tertiary Care Hospital at Jhalawar, Rajasthan, India. J ClinDiagn Res 2012;6:182-7.
5. Sharma M, Pathak S, Srivastava P. Prevalence and antibiogram of Extended Spectrum β-Lactamase (ESBL) producing Gram negative bacilli and furthermolecular characterization of ESBL producing Escherichia coli and Klebsiella sppAntibiogram and Molecular Characterization of ESBL Producers. J ClinDiagn Res 2013;7:2173-7.
6. Tsering DC, Das S, Adhiakari L, Pal R, Singh TS. Extended spectrum β-lactamase detection in gram negative bacilli of nosocomial origin. J Glob infect Dis 2009;1:87-92. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
7. Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing. Twentieth informational supplement ed. CLSI document M100-S20. Wayne, PA:CLSI; 2010.
8. Jonathan N. Screening for extended-spectrum Beta-Lactamase-producing pathogenic enterobacteria in district general hospitals. J ClinMicrobiol 2005;43:1488–90. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
9. Afridi FI, Farooqui BJ, Hussain A. Frequency of Extended Spectrum Beta Lactamase Producing EnterobacteriaceaeAmong Urinary Pathogen Isolates. J Col PhysicansSurg Pak 2011;21:741-2. [Pubmed]   
10. Agrawal P, Gosh AN, Satish K, Basu B, Kapila K. Prevalence of extended-spectrum β-lactamases among Escherichia coli and Klebsiella pneumoniae isolates in a tertiary care hospital. Indian J PatholMicrobiol 2008;51:139-42. [DOI via Crossref]   
11. Anathakrishnan AN, Kanungo R, Kumar A, Badrinath S. Detection of Extended Spectrum β-lactamases producers among surgical wound infections and burn patients in JIPMER. Indian J Med Microbiol 2000;18:160-5.
12. Kumar MS, Lakshmi V, Rajgoplan R. Occurrence of Extended spectrum β-lactamases among Enterobacteriaceae species isolated at teriatry care Institute. Indian J Med Microbiol 2006;24:208-2. [Pubmed]   
13. Amita J, Indranil R, Mahendra KG, Kumar M, Agarwal SK. Prevalence of extended-spectrum β-lactamase producing Gram-negative bacteria in septicaemic neonates in a tertiary care hospital. J Med Microbiol 2003;52:421-5. [DOI via Crossref]   

How to Cite this Article
Pubmed Style

Bhavanarushi Sreekanth, Gogi Suresh Dattaraya. Detection of extended spectrum β-lactamases (ESBLS) producers in clinical isolates. Int J Med Sci Public Health. 2014; 3(9): 1132-1134. doi:10.5455/ijmsph.2014.010720142


Web Style

Bhavanarushi Sreekanth, Gogi Suresh Dattaraya. Detection of extended spectrum β-lactamases (ESBLS) producers in clinical isolates. http://www.ijmsph.com/?mno=161163 [Access: August 24, 2019]. doi:10.5455/ijmsph.2014.010720142


AMA (American Medical Association) Style

Bhavanarushi Sreekanth, Gogi Suresh Dattaraya. Detection of extended spectrum β-lactamases (ESBLS) producers in clinical isolates. Int J Med Sci Public Health. 2014; 3(9): 1132-1134. doi:10.5455/ijmsph.2014.010720142



Vancouver/ICMJE Style

Bhavanarushi Sreekanth, Gogi Suresh Dattaraya. Detection of extended spectrum β-lactamases (ESBLS) producers in clinical isolates. Int J Med Sci Public Health. (2014), [cited August 24, 2019]; 3(9): 1132-1134. doi:10.5455/ijmsph.2014.010720142



Harvard Style

Bhavanarushi Sreekanth, Gogi Suresh Dattaraya (2014) Detection of extended spectrum β-lactamases (ESBLS) producers in clinical isolates. Int J Med Sci Public Health, 3 (9), 1132-1134. doi:10.5455/ijmsph.2014.010720142



Turabian Style

Bhavanarushi Sreekanth, Gogi Suresh Dattaraya. 2014. Detection of extended spectrum β-lactamases (ESBLS) producers in clinical isolates. International Journal of Medical Science and Public Health, 3 (9), 1132-1134. doi:10.5455/ijmsph.2014.010720142



Chicago Style

Bhavanarushi Sreekanth, Gogi Suresh Dattaraya. "Detection of extended spectrum β-lactamases (ESBLS) producers in clinical isolates." International Journal of Medical Science and Public Health 3 (2014), 1132-1134. doi:10.5455/ijmsph.2014.010720142



MLA (The Modern Language Association) Style

Bhavanarushi Sreekanth, Gogi Suresh Dattaraya. "Detection of extended spectrum β-lactamases (ESBLS) producers in clinical isolates." International Journal of Medical Science and Public Health 3.9 (2014), 1132-1134. Print. doi:10.5455/ijmsph.2014.010720142



APA (American Psychological Association) Style

Bhavanarushi Sreekanth, Gogi Suresh Dattaraya (2014) Detection of extended spectrum β-lactamases (ESBLS) producers in clinical isolates. International Journal of Medical Science and Public Health, 3 (9), 1132-1134. doi:10.5455/ijmsph.2014.010720142





Most Viewed Articles
  • Management of Appendicular Lump: Early exploration Vs conservative management
    Chandra Prakash Pandey, Ram Chandra Kesharwani, Chandra Gopal Singh Chauhan, Manmohan Krishna Pandey, Purnima Mittra, Pramod Kumar, Arshad Raza
    Int J Med Sci Public Health. 2013; 2(4): 1046-1049
    » Abstract & References » doi: 10.5455/ijmsph.2013.230820131

  • Internet addiction among professional courses students: A study from central India
    Arvind Sharma, Rupesh Sahu, Pradeep Kumar Kasar, Richa Sharma
    Int J Med Sci Public Health. 2014; 3(9): 1069-1073
    » Abstract & References » doi: 10.5455/ijmsph.2014.180620142

  • Burden of non communicable diseases in India: Setting priority for action
    Kalpa Sharma
    Int J Med Sci Public Health. 2013; 2(1): 7-11
    » Abstract & References » doi: 10.5455/ijmsph.2013.2.7-11

  • Diabetic nephropathy: Progression and pathophysiology
    Deepak N Parchwani, Amit A Upadhyah
    Int J Med Sci Public Health. 2012; 1(2): 59-70
    » Abstract & References » doi: 10.5455/ijmsph.2012.1.59-70

  • Elevated levels of serum uric acid, creatinine or urea in preeclamptic women
    Magna Manjareeka, Sitikantha Nanda
    Int J Med Sci Public Health. 2013; 2(1): 43-47
    » Abstract & References » doi: 10.5455/ijmsph.2013.2.43-47

  • Maternal and fetal outcome in oligohydramnios- Study of 100 cases
    Krishna Jagatia, Nisha Singh, Sachin Patel
    Int J Med Sci Public Health. 2013; 2(3): 724-727
    » Abstract & References » doi: 10.5455/ijmsph.2013.070520132

  • Zinc: The trace element of major importance in human nutrition and health
    Jayant D Deshpande, Mohini M Joshi, Purushottam A Giri
    Int J Med Sci Public Health. 2013; 2(1): 1-6
    » Abstract & References » doi: 10.5455/ijmsph.2013.2.1-6

  • Knowledge, Attitude, and Practices (KAP) study on HIV / AIDS among HIV patients, care givers and general population in north-eastern part of India.
    L P Meena, S K Pandey, M Rai, Anju Bharti, Shyam Sunder
    Int J Med Sci Public Health. 2013; 2(1): 36-42
    » Abstract & References » doi: 10.5455/ijmsph.2013.2.36-42

  • Patient satisfaction about health care services: A cross sectional study of patients who visit the outpatient department of a civil hospital at Surendranagar, Gujarat
    Krupal Joshi, Kishor Sochaliya, Shyamal Purani, Girija Kartha
    Int J Med Sci Public Health. 2013; 2(3): 659-663
    » Abstract & References » doi: 10.5455/ijmsph.2013.250420131

  • Prevalence and pattern of substance abuse among school childern in nothern India: A rapid assesment study
    Syed Shuja Qadri, RKD Goel, Jagjeet Singh, Surendra Kumar Ahluwalia, Rambha Pathak, Humaira Bashir
    Int J Med Sci Public Health. 2013; 2(2): 273-282
    » Abstract & References » doi: 10.5455/ijmsph.2013.2.271-280

  • Most Downloaded
  • Minor disorder of pregnancy and its home management
    Lata Gururani, Atul Kumar, Gomathi Mahalingam
    Int J Med Sci Public Health. 2016; 5(4): 684-687
    » Abstract & References » doi: 10.5455/ijmsph.2016.03082015111

  • Association between serum ferritin and thyroid hormone profile in hypothyroidism
    Ashuma Sachdeva, Veena Singh, Isha Malik, Prasanta Saha Roy, Himanshu Madaan, Rajesh Nair
    Int J Med Sci Public Health. 2015; 4(6): 863-865
    » Abstract & References » doi: 10.5455/ijmsph.2015.24012015174

  • Internet addiction among professional courses students: A study from central India
    Arvind Sharma, Rupesh Sahu, Pradeep Kumar Kasar, Richa Sharma
    Int J Med Sci Public Health. 2014; 3(9): 1069-1073
    » Abstract & References » doi: 10.5455/ijmsph.2014.180620142

  • Effect of sleep deprivation on the attitude and performance of medical students, Riyadh, Saudi Arabia
    Hussein Saad Amin, Ibrahim Salamah Almazroua, Abdullah Sulaiman Alsahlan, Mohammed Abdullah Alrishan, Houssam Mourad Elmourad, Mohammed Mansour Alotaibi, Abdulaziz Ibrahim Almohaisin
    Int J Med Sci Public Health. 2016; 5(3): 575-580
    » Abstract & References » doi: 10.5455/ijmsph.2016.17112015207

  • Management of Appendicular Lump: Early exploration Vs conservative management
    Chandra Prakash Pandey, Ram Chandra Kesharwani, Chandra Gopal Singh Chauhan, Manmohan Krishna Pandey, Purnima Mittra, Pramod Kumar, Arshad Raza
    Int J Med Sci Public Health. 2013; 2(4): 1046-1049
    » Abstract & References » doi: 10.5455/ijmsph.2013.230820131

  • Epidemiology and prevalence of dermatological diseases among schoolchildren of Medak district, Telanganaa clinical survey
    Lakshmi Kumari Villa, Gopi Krishna
    Int J Med Sci Public Health. 2016; 5(7): 1475-1478
    » Abstract & References » doi: 10.5455/ijmsph.2016.28102015238

  • Gunthers with haemolytic anaemia extremly rare: A case report
    Nilesh Kumar, Ankur Varshney, Arvind Anand, Dibyaranjan Behera, Ravi Anand, Ashutosh Tiwari
    Int J Med Sci Public Health. 2013; 2(2): 472-474
    » Abstract & References » doi: 10.5455/ijmsph.2013.2.313-315

  • Diabetic nephropathy: Progression and pathophysiology
    Deepak N Parchwani, Amit A Upadhyah
    Int J Med Sci Public Health. 2012; 1(2): 59-70
    » Abstract & References » doi: 10.5455/ijmsph.2012.1.59-70

  • Patient satisfaction about health care services: A cross sectional study of patients who visit the outpatient department of a civil hospital at Surendranagar, Gujarat
    Krupal Joshi, Kishor Sochaliya, Shyamal Purani, Girija Kartha
    Int J Med Sci Public Health. 2013; 2(3): 659-663
    » Abstract & References » doi: 10.5455/ijmsph.2013.250420131

  • Burden of non communicable diseases in India: Setting priority for action
    Kalpa Sharma
    Int J Med Sci Public Health. 2013; 2(1): 7-11
    » Abstract & References » doi: 10.5455/ijmsph.2013.2.7-11

  • Most Cited Articles
  • Zinc: The trace element of major importance in human nutrition and health
    Jayant D Deshpande, Mohini M Joshi, Purushottam A Giri
    Int J Med Sci Public Health. 2013; 2(1): 1-6
    » Abstract & References » doi: 10.5455/ijmsph.2013.2.1-6
    Cited : 25 times [Click to see citing articles]

  • Burden of non communicable diseases in India: Setting priority for action
    Kalpa Sharma
    Int J Med Sci Public Health. 2013; 2(1): 7-11
    » Abstract & References » doi: 10.5455/ijmsph.2013.2.7-11
    Cited : 13 times [Click to see citing articles]

  • Design of fuzzy expert system for diagnosis of cardiac diseases
    Smita Sushil Sikchi , Sushil Sikchi , M S ALI
    Int J Med Sci Public Health. 2013; 2(1): 56-61
    » Abstract & References » doi: 10.5455/ijmsph.2013.2.56-61
    Cited : 9 times [Click to see citing articles]

  • Sugammadex associate persistant bradycardia
    Murat Bilgi, Abdullah Demirhan, Akcan Akkaya, Umit Yasar Tekelioglu, Hasan Kocoglu
    Int J Med Sci Public Health. 2014; 3(3): 372-374
    » Abstract & References » doi: 10.5455/ijmsph.2013.251220131
    Cited : 8 times [Click to see citing articles]

  • Migration and health-care access: Barriers to access government health services by migrant tribal community living in an eastern Indian city
    Suchismita Mishra, Yadlapalli S Kusuma, Bontha V Babu
    Int J Med Sci Public Health. 2015; 4(1): 101-108
    » Abstract & References » doi: 10.5455/ijmsph.2015.0110201424
    Cited : 7 times [Click to see citing articles]

  • Clinico-microbiological profile of women with vaginal discharge in a tertiary care hospital of northern India
    Varsha Chaudhary, Ved Prakesh, Kian Agarwal, Vijender Kumar Agrawal, Arun Singh, Sachin Pandey
    Int J Med Sci Public Health. 2012; 1(2): 75-80
    » Abstract & References » doi: 10.5455/ijmsph.2012.1.75-80
    Cited : 6 times [Click to see citing articles]

  • Correlation between obesity and cardio respiratory fitness
    Prabha Setty, BV Padmanabha, BR Doddamani
    Int J Med Sci Public Health. 2013; 2(2): 300-304
    » Abstract & References » doi: 10.5455/ijmsph.2013.2.298-302
    Cited : 6 times [Click to see citing articles]

  • Knowledge, Attitude, and Practices (KAP) study on HIV / AIDS among HIV patients, care givers and general population in north-eastern part of India.
    L P Meena, S K Pandey, M Rai, Anju Bharti, Shyam Sunder
    Int J Med Sci Public Health. 2013; 2(1): 36-42
    » Abstract & References » doi: 10.5455/ijmsph.2013.2.36-42
    Cited : 6 times [Click to see citing articles]

  • Non albicans Candida species: its isolation pattern, species distribution, virulence factors and antifungal susceptibility profile
    Sachin Chandrakant Deorukhkar, Santosh Saini
    Int J Med Sci Public Health. 2013; 2(3): 533-538
    » Abstract & References » doi: 10.5455/ijmsph.2013.080320131
    Cited : 6 times [Click to see citing articles]

  • Initial default among tuberculosis patients diagnosed in selected medical colleges of Puducherry: issues and possible interventions
    Divija Pillai, Anil J Purty, Stalin Prabakaran, Zile Singh, Govindarajan Soundappan, Velavan Anandan
    Int J Med Sci Public Health. 2015; 4(7): 957-960
    » Abstract & References » doi: 10.5455/ijmsph.2015.30012015196
    Cited : 6 times [Click to see citing articles]